Jaruwan WongthanateChongrak Polprasertจารุวรรณ วงค์ทะเนตรMahidol University. Faculty of Environment and Resource Studies.Thammasat University. Faculty of Engineering. Department of2016-02-062020-01-072016-02-062020-01-072016-02-082015-01Braz. Arch. Biol. Technol. Vol. 58, No. 1 (2015). 124-130.1516-8913https://repository.li.mahidol.ac.th/handle/20.500.14594/48740Biohydrogen production was studied from the vermicelli processing wastewater using synthetic and biological materials as immobilizing substrate employing a mixed culture in a batch reactor operated at the initial pH 6.0 and thermophilic condition (55 ± 1ºC). Maximum cumulative hydrogen production (1,210 mL H2/L wastewater) was observed at 5% (v/v) addition of ring-shaped synthetic material, which was the ring-shaped hydrophobic acrylic. Regarding 5% (v/v) addition of synthetic and biological materials, the maximum cumulative hydrogen production using immobilizing synthetic material of ball-shaped hydrophobic polyethylene (HBPE) (1,256.5 mL H2/L wastewater) was a two-fold increase of cumulative hydrogen production when compared to its production using immobilizing biological material of rope-shaped hydrophilic ramie (609.8 mL H2/L wastewater). SEM observation of immobilized biofilm on a ball-shaped HBPE or a rope-shaped hydrophilic ramie was the rod shape and gathered into group.engMahidol University.Biohydrogen productionBiofilmSynthetic materialBiological materialSEMArticlehttp://dx.doi.org/10.1590/S1516-8913201502895