Harms K.Paomephan P.Boonpratuang T.Choeyklin R.Boonchird C.Surup F.Mahidol University2023-12-112023-12-112023-01-01Journal of Natural Products (2023)01633864https://repository.li.mahidol.ac.th/handle/20.500.14594/91395Metabolites 1 and 2, isolated from cultures of the basidiomycete Resupinatus sp. BCC84615, collected in a tropical forest in northeastern Thailand, showed weak antibiotic activity against Bacillus subtilis and Staphylococcus aureus and cytotoxicity against cancer cell lines. Their planar structures were elucidated by high-resolution electrospray ionization mass spectrometry and NMR spectroscopy as clavilactone J, known from the basidiomycete Ampulloclitocybe clavipes, and its new 1,4-benzoquinone derivative. A detailed analysis of the ROESY correlations in 1 confirmed the recent revision of the relative configuration of clavilactone J. However, specific rotation and Cotton effects observed by electronic circular dichroism were contrary to those of the clavilactones; thus, we assigned a rare antipodal absolute configuration.ChemistryArticleSCOPUS10.1021/acs.jnatprod.3c001742-s2.0-851782476031520602537931226