Genetic differences of E gene of A Thai strain of Japanese encephalitis virus that determine small plaque size phenotype but not neurovirulence in suckling mice

Sasithorn LorroengsilAntony ChenMatti SällbergSomsak PantuwatanaMahidol UniversityUbon Rajathanee UniversityKarolinska InstitutetBurapha University2018-07-122018-07-122008-05-01Southeast Asian Journal of Tropical Medicine and Public Health. Vol.39, No.3 (2008), 387-393012515622-s2.0-44949179422https://repository.li.mahidol.ac.th/handle/20.500.14594/19690Two small plaque variants of Japanese encephalitis virus (JEV), S4P9 and S9P10, were recovered from the wild type of JEV strain KE-093 using plaque purification in combination with the temperature-shift induction technique. Growth patterns of the S4P9 and S9P10 in BHK-21 cells as well as neurovirulence in suckling mice were similar to that of KE-093. An amino acid substitution, lysine for glutamic acid, was present in envelope protein at residue E-83 in the small plaque variants. This study shows that small plaque phenotype is not always associated with attenuation in vivo.Mahidol UniversityMedicineGenetic differences of E gene of A Thai strain of Japanese encephalitis virus that determine small plaque size phenotype but not neurovirulence in suckling miceArticleSCOPUS