PBS incubation, a simple technique to release miracidia of Opisthorchis-like eggs for DNA extraction

Saiwasan BuathongMathirut MungthinPeerapan Tan-ariyaTawee NaaglorPaanjit TaamasriPicha SuwannahitatornSaovanee LeelayoovaMahidol UniversityPhramongkutklao College of Medicine2018-09-242018-09-242010-03-01Experimental Parasitology. Vol.124, No.3 (2010), 357-35910902449001448942-s2.0-76049100690https://repository.li.mahidol.ac.th/handle/20.500.14594/29254This study describes a simple technique for releasing miracidia from Opisthorchis-like eggs before DNA extraction by keeping PBS washed specimens at 4°C for two days. Using PCR amplification, the sensitivity of the PBS incubation technique was as good as that obtained from freeze-thaw technique. Moreover, this low-cost technique is less laborious. © 2009 Elsevier Inc.Mahidol UniversityImmunology and MicrobiologyPBS incubation, a simple technique to release miracidia of Opisthorchis-like eggs for DNA extractionArticleSCOPUS10.1016/j.exppara.2009.11.011