Olivier GoupilleTipparat PenglongZahra KadriMarine Granger-LocatelliRaphaël DenisSerge LuquetCécile BadoualSuthat FucharoenLeila Maouche-ChrétienPhilippe LeboulchStany ChrétienUniversite Paris-SaclayMahidol UniversityUniversite Paris 7- Denis DiderotHopital Europeen Georges-PompidouInsermBrigham and Women's Hospital and Harvard Medical School2018-12-212019-03-142018-12-212019-03-142017-12-19Cell Reports. Vol.21, No.12 (2017), 3524-3535221112472-s2.0-85039946205https://repository.li.mahidol.ac.th/handle/20.500.14594/41664© 2017 The Author(s) GATA transcription factors and their FOG cofactors play a key role in tissue-specific development and differentiation, from worms to humans. Mammals have six GATA and two FOG factors. We recently demonstrated that interactions between retinoblastoma protein (pRb) and GATA-1 are crucial for erythroid proliferation and differentiation. We show here that the LXCXE pRb-binding site of FOG-2 is involved in adipogenesis. Unlike GATA-1, which inhibits cell division, FOG-2 promotes proliferation. Mice with a knockin of a Fog2 gene bearing a mutated LXCXE pRb-binding site are resistant to obesity and display higher rates of white-to-brown fat conversion. Thus, each component of the GATA/FOG complex (GATA-1 and FOG-2) is involved in pRb/E2F regulation, but these molecules have markedly different roles in the control of tissue homeostasis. Goupille et al. find that a mutation of the FOG-2 LXCXE pRb-binding site decreases cell proliferation and affects adipogenesis in vitro and in vivo. Fog2Rb−/Rb−mutant mice are resistant to obesity, and they present abnormal WAT/BAT conversion and lactate production. Oxamate treatment results in phenotype reversion in these mice.Mahidol UniversityBiochemistry, Genetics and Molecular BiologyArticleSCOPUS10.1016/j.celrep.2017.11.098