Banthit ChetsawangJirapa ChetsawangPiyarat GovitrapongThe Institute of Science and Technology for Research and Development, Mahidol UniversityMahidol University2018-09-132018-09-132009-01-01Journal of Pineal Research. Vol.46, No.1 (2009), 36-421600079X074230982-s2.0-57149133830https://repository.li.mahidol.ac.th/handle/20.500.14594/27324Neuroprotective effects of melatonin against oxidative stress-induced neuronal cell degeneration in human SH-SY5Y neuroblastoma cells were investigated in this report. The results demonstrate that exogenous administration of H2O2 and 1-methyl, 4-phenyl, pyridinium ion (MPP+) significantly decreased cell viability in SH-SY5Y cultured cells. Desipramine, a monoamine uptake blocker was able to abolish the toxic effects of MPP+ but not H2O2 in reduction of cell viability. Conversely, melatonin reversed the toxic effects of H 2O2 and MPP+ on cell viability. In addition, the reduction of phosphorylation of tyrosine hydroxylase, the rate limiting enzyme in dopamine synthesis, and phosphorylation of cyclic AMP responsive element-binding protein by H2O2 and MPP+ was also diminished by melatonin. These results demonstrate some effective roles of melatonin on neuroprotection and its action on the modulation of tyrosine hydroxylase phosphorylation. © 2008 The Authors.Mahidol UniversityBiochemistry, Genetics and Molecular BiologyArticleSCOPUS10.1111/j.1600-079X.2008.00605.x