Somsin PetyimChanon NeungtonIsarin ThanaboonyawatPitak LaokirkkiatRoungsin ChoavaratanaMahidol University2018-11-092018-11-092014-01-01Journal of Assisted Reproduction and Genetics. Vol.31, No.12 (2014), 1673-168015737330105804682-s2.0-84914159575https://repository.li.mahidol.ac.th/handle/20.500.14594/33346© 2014, Springer Science+Business Media New York. Purpose: To evaluate whether sperm preparation (swim-up technique) before freezing improves the percentages of sperm motility, sperm viability, and non-apoptotic spermatozoa after freezing-thawing process compared with preparation after cryopreservation.Methods: Semen samples from 65 infertile males were equally divided into two aliquots one of which was processed for swim-up prior to cryopreservation and one of which was processed following cryopreservation. Sperm count, motility, and apoptosis index were measured in each group.Result (s): The total sperm count and the total motile sperm count decreased after thawing in both the pre-preparation and non-preparation groups compared with neat semen group (P < 0.001). Moreover, the percentage of apoptotic sperm in the pre-preparation group after cryopreservation was lower than that in the non-preparation group (P < 0.05), whereas the percentage of vital sperm with progressive motility was higher than that in the pre-preparation group (P < 0.001).Conclusion (s): Semen preparation by swim-up before freezing resulted in better sperm quality and fewer apoptotic sperm than sperm preparation after thawing. Therefore, sperm preparation before cryopreservation should be considered in routine sperm cryopreservation.Mahidol UniversityBiochemistry, Genetics and Molecular BiologyMedicineArticleSCOPUS10.1007/s10815-014-0332-y