Techakasikornpanich M.Jangpatarapongsa K.Polpanich D.Zine N.Errachid A.Elaissari A.Mahidol University2024-09-092024-09-092024-11-01TrAC - Trends in Analytical Chemistry Vol.180 (2024)01659936https://repository.li.mahidol.ac.th/handle/20.500.14594/101155Foodborne diseases are a global concern due to their high impact on morbidity, and mortality worldwide. The traditional method of bacterial detection is both time-consuming and labor-intensive. Alternative methods, such as immunoassays and polymerase chain reactions, have limited applicability due to the need for an enrichment process and expensive equipment. DNA-based biosensors have gained interest in the field of detection, due to their high rapidity, high sensitivity, and high specificity, which provides a promising technology for bacterial detection. To fabricate DNA-based biosensors, the immobilization of DNA probes on electrodes is a crucial process. This review delves into the three techniques of DNA probe immobilization, discussing their respective advantages and disadvantages. Furthermore, recent advancements in DNA-based biosensors are explored. The integration of advanced molecular techniques such as DNA nanostructures, DNA walkers, and the CRISPR-Cas system with DNA-based biosensors for foodborne pathogens is also discussed in this work.ChemistryReviewSCOPUS10.1016/j.trac.2024.1179252-s2.0-8520301452018793142