Emmanuelle GorichonCorinne MartinKesara Na BangchangJuntra KarbwangAlain ThuillierRobert FarinottiFrançois GimenezHopital Pitie SalpetriereMahidol UniversityUniversite Paris-Sud XI2018-07-042018-07-041998-08-07Journal of Chromatography B: Biomedical Applications. Vol.712, No.1-2 (1998), 259-262157264952-s2.0-0032493708https://repository.li.mahidol.ac.th/handle/20.500.14594/18342We describe a direct liquid chromatographic method with spectrofluorimetric detection to quantify the two enantiomers of halofantrine and the two enantiomers of its main chiral N-monodesbutylated metabolite in erythrocyte pellets. The method involves a Chiralpak AD column and a rapid one-step extraction procedure with acetonitrile. The method was validated for the four enantiomers within the range 0-1000 ng/ml. The absence of stereoconversion was studied in samples stored frozen for up to eight months. The optical rotation of the halofantrine and metabolite enantiomers was determined after separation on a semi-preparative Chiralcel OD column with polarimetric detection.Mahidol UniversityChemistryArticleSCOPUS10.1016/S0378-4347(98)00151-0