Muhammad Hatta PrabowoSupawat ChatchenPatsamon RijiravanichPana KlamkamThanit ChalermwatanachaiKriengsak LimkittikulWerasak SurareungchaiFaculty of Tropical Medicine, Mahidol UniversityUniversitas Islam IndonesiaKing Mongkut's University of Technology ThonburiPhramongkutklao College of Medicine2022-08-042022-08-042021-06-01International Journal of Infectious Diseases. Vol.107, (2021), 271-27718783511120197122-s2.0-85106513084https://repository.li.mahidol.ac.th/handle/20.500.14594/78164Objectives: This study aimed to evaluate a microfluidic paper-based analytical device (DEN-NS1-PAD) based on a rapid NS1 antigen test for diagnosing dengue at the point of care. Methods: 219 serum samples from suspected dengue cases were tested with the developed DEN-NS1-PAD and commercial RDT by SD BIOLINE. The results were compared with the nested-PCR results. Results: The limit of detection of DEN-NS1-PAD was 0.78 ng mL−1. It showed 88.89% sensitivity, 86.67% specificity, and a substantial agreement correlation (κ = 0.7522) compared with nested-PCR. In contrast, SD BIOLINE for NS1 (SD-NS1) detection showed 87.88% sensitivity, 90.00% specificity, and had a substantial agreement correlation with nested-PCR (κ = 0.7788). Conclusions: DEN-NS1-PAD is a valuable tool for diagnosing DENV infections, especially for diagnosed patients with early acute phase samples with high viral load. DEN-NS1-PAD has better sensitivity than SD-NS1 but less specificity.Mahidol UniversityMedicineArticleSCOPUS10.1016/j.ijid.2021.05.007