Pongsakorn ThawonpanSupinya ThanapongpichatWatthanachai JumpathongAung Win TunChurat WeeraphanWorrawit WanitsuwanHansuk BuncherdChulabhorn Research InstituteChulabhorn Royal AcademyMahidol UniversityPrince of Songkla University2022-08-042022-08-042021-09-01Songklanakarin Journal of Science and Technology. Vol.43, No.5 (2021), 1443-1448012533952-s2.0-85122289106https://repository.li.mahidol.ac.th/handle/20.500.14594/79305This work focuses on the separation of diethylpyrocarbonate-modified histidine-containing peptides by strong cation exchange (SCX) chromatography prior to mass spectrometric analysis. The decrease in positive charge of diethylpyrocarbonate-treated imidazole in histidine, masked by the incorporation of the carbethoxyl group, provides us with an opportunity to use SCX for separating diethylpyrocarbonate-modified histidine-containing peptides from the unmodified ones. In this report, the diethylpyrocarbonate-modified histidine-containing peptide is eluted faster than the untreated peptide. Furthermore, the carbethoxy group can be effectively removed to generate the unmodified imidazole in the histidine residue by neutral hydroxylamine.Mahidol UniversityMultidisciplinaryArticleSCOPUS