Charoenwongphaibun C.Lorthongpanich C.Septham P.Wangpaiboon K.Panpetch P.Pichyangkura R.Charoenwongpaiboon T.Kuttiyawong K.Mahidol University2023-05-192023-05-192023-03-01Gels Vol.9 No.3 (2023)https://repository.li.mahidol.ac.th/handle/20.500.14594/81708Levan is a biopolymer composed of fructose chains covalently linked by β−2,6 glycosidic linkages. This polymer self−assembles into a nanoparticle of uniform size, making it useful for a wide range of applications. Also, levan exhibits various biological activities such as antioxidants, anti-inflammatory, and anti-tumor, that make this polymer very attractive for biomedical application. In this study, levan synthesized from Erwinia tasmaniensis was chemically modified by glycidyl trimethylammonium chloride (GTMAC) to produce cationized nanolevan (QA-levan). The structure of the obtained GTMAC−modified levan was determined by FT-IR, 1H-NMR and elemental (CHN) analyzer. The size of the nanoparticle was calculated using the dynamic light scattering method (DLS). The formation of DNA/QA-levan polyplex was then investigated by gel electrophoresis. The modified levan was able to increase the solubility of quercetin and curcumin by 11-folds and 205-folds, respectively, compared to free compounds. Cytotoxicity of levan and QA−levan was also investigated in HEK293 cells. This finding suggests that GTMAC−modified levan should have a potential application for drug and nucleic acid delivery.Chemical EngineeringArticleSCOPUS10.3390/gels90301882-s2.0-8515128744023102861