Theerasak JuagwonChutiparn LertvachirapaiboonKazunari ShinboKeizo KatoToemsak SrikhirinTanakorn OsotchanAkira BabaNiigata UniversityMahidol University2020-01-272020-01-272019-02-01IEICE Transactions on Electronics. Vol.E102C, No.2 (2019), 125-13117451353091685242-s2.0-85061122639https://repository.li.mahidol.ac.th/handle/20.500.14594/50862Copyright © 2019 The Institute of Electronics, Information and Communication Engineers. In this work, we report the in situ growth of gold nanoparticles (AuNPs) for the improvement of a transmission surface plasmon resonance (T-SPR) sensor to detect human immunoglobulin G (IgG). Human IgG was immobilized on an activated self-assembled monolayer of 11-mercaptoundecanoic on a gold-coated grating substrate. The T-SPR system was also used to monitor the construction of sensor chips as well as the binding of IgG and anti-IgG conjugated with AuNPs. After specific adsorption with IgG, the T-SPR signal was further enhanced by the in situ growth of AuNPs bound with anti-IgG. Using AuNP conjugation and in situ growth of bound AuNPs, the sensitivity of the IgG immunosensor was improved by two orders of magnitude compared with that without conjugated AuNPs.Mahidol UniversityEngineeringMaterials ScienceArticleSCOPUS10.1587/transele.2018OMP0004