Establishing of human induced pluripotent stem cell line DMSCi002-A from the hematopoietic stem cells of a healthy male donor

Bumroongthai K.Yodtup C.Jantapalaboon D.Phairoh P.Suparak S.Dhepakson P.Tubsuwan A.Wongkidakarn S.Mahidol University2025-01-232025-01-232025-03-01Stem Cell Research Vol.83 (2025)18735061https://repository.li.mahidol.ac.th/handle/20.500.14594/102945Using the integration-free episomal vector containing the reprogramming components OCT3/4/shp53, Sox2/KLF4, L-MYC/LIN28, and EBNA-1, hematopoietic stem cells obtained from a healthy 33-year-old man were effectively reprogrammed and turned into induced pluripotent stem cells (iPSCs). The reprogrammed iPSCs were grown without the use of feeders. They exhibited a normal karyotype, displayed pluripotency markers, and differentiated into cells from the three germ layers. This DMSCi002-A line may serve as a control for investigating disease mechanisms.Biochemistry, Genetics and Molecular BiologyEstablishing of human induced pluripotent stem cell line DMSCi002-A from the hematopoietic stem cells of a healthy male donorArticleSCOPUS10.1016/j.scr.2025.1036552-s2.0-8521429934318767753