Chukiat SirivichayakulArunee SabchareonKriengsak LimkittikulSutee YoksanMahidol University2018-11-092018-11-092014-03-12Virology Journal. Vol.11, No.1 (2014)1743422X2-s2.0-84898422509https://repository.li.mahidol.ac.th/handle/20.500.14594/33983Background: The plaque reduction neutralization test (PRNT) is currently the best and most widely accepted approach to measuring virus-neutralizing and protective antibodies to dengue virus, and in assessing the immunogenicity of a dengue vaccine. However, the correlation between presence of dengue-neutralizing antibody and protection from infection is not absolute. Findings. In a cohort study in Ratchaburi Province, Thailand, 48 subjects with serologically confirmed symptomatic dengue infection were tested for pre-existing dengue neutralizing antibody using PRNT. Nine subjects had quite high pre-existing PRNT50 titers (titer >90) to subsequent infecting dengue serotypes, but still had symptomatic infections. Conclusion: This report provides evidence that PRNT may not be a good test for predicting protection against subsequent dengue infection. © 2014Sirivichayakul et al.; licensee BioMed Central Ltd.Mahidol UniversityImmunology and MicrobiologyMedicineArticleSCOPUS10.1186/1743-422X-11-48