Adul DulsukSuporn PaksanontAdisak SangchankoomPeeraya EkchariyawatRungnapa PhunpangYaowaruk JutrakulNarisara ChantratitaT. Eoin WestMahidol UniversityUdon Thani Regional Medical Sciences CenterUniversity of Washington, Seattle2018-12-112019-03-142018-12-112019-03-142016-01-01Transactions of the Royal Society of Tropical Medicine and Hygiene. Vol.110, No.11 (2016), 670-67218783503003592032-s2.0-85014381547https://repository.li.mahidol.ac.th/handle/20.500.14594/40858© The Author 2016. Background: Identification of Burkholderia pseudomallei, the cause of melioidosis, using routine methods takes several days. Use of a monoclonal antibody-based immunofluorescent assay (IFA) on positive blood cultures may speed diagnosis. Methods: We tested the diagnostic accuracy of the IFA on 545 blood cultures positive for Gram-negative organisms at Udon Thani Hospital, Thailand, between June 2015 and August 2016. Results: Sensitivity of the IFA was 100% and specificity was 99.6%. The median decrease in time to pathogen identification between the IFA result and routine methods was 28 h (IQR 25-51), p < 0.0001. Conclusions: The IFA accurately expedites the diagnosis of melioidosis.Mahidol UniversityImmunology and MicrobiologyArticleSCOPUS