Tadahiro SasakiItaru HiraiMarie NikiTatsuya NakamuraChalit KomalamisraWanna MaipanichTeera KusolsukSurapol Sa-nguankiatSomchit PubampenYoshimasa YamamotoOsaka University Faculty of MedicineKobe University School of MedicineKansai Medical UniversityMahidol University2018-09-242018-09-242010-01-27Journal of Antimicrobial Chemotherapy. Vol.65, No.4 (2010), 666-66814602091030574532-s2.0-77952565591https://repository.li.mahidol.ac.th/handle/20.500.14594/29792Objectives: To determine the prevalence of CTX-M β-lactamase-producing Enterobacteriaceae in stool specimens obtained from healthy individuals in a rural area of Thailand. Methods: Bacteria in stool specimens were screened for extended-spectrum β-lactamase (ESBL) production on McConkey agar with cefotaxime and confirmed by the double-disc synergy test. Genetic detection and genotyping of CTX-M-type ESBL was performed by PCR with bacterial DNA extracted from isolates. Results: A markedly high number (82 of 141, 58.2%) of the specimens showed the presence of CTX-M β-lactamase-producing Enterobacteriaceae, as confirmed by both phenotypic and genetic examinations. The majority of the CTX-M β-lactamase-producing bacteria were Escherichia coli (85.1%). Conclusions: The study revealed the wide dissemination of CTX-M β-lactamase-producing Enterobacteriaceae in the healthy population. © The Author 2010. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy.Mahidol UniversityMedicinePharmacology, Toxicology and PharmaceuticsArticleSCOPUS10.1093/jac/dkq008