Witoon TirasophonMathurose PonglikitmongkolPrapon WilairatVichai BoonsaengSakol PanyimMahidol University2018-08-102018-08-101991-02-28Biochemical and Biophysical Research Communications. Vol.175, No.1 (1991), 179-184109021040006291X2-s2.0-0026036365https://repository.li.mahidol.ac.th/handle/20.500.14594/21992Detection of Plasmodium falciparum malaria by a specific DNA probe is a highly promising means for epidemiological surveillance of human malaria. However, none of presently available DNA probe methods could detect as little as a few parasites in infected blood. By amplification of a specific 206 base pairs P. falciparum DNA sequence using the polymerase chain reaction (PCR), as little as 0.01 picogram DNA or one-half of a parasite was sufficient for a specific detection. A PCR procedure for detection of P. falciparum in infected blood without prior DNA extraction was also developed which was sensitive for a single parasite. The procedure was simple and should be applicable for a large scale epidemiological study involving a very low parasitemia situation. © 1991 Academic Press, Inc.Mahidol UniversityBiochemistry, Genetics and Molecular BiologyArticleSCOPUS10.1016/S0006-291X(05)81217-3