Norasuthi BangphoomiAkiko Takenaka-UemaTatsuki SugiKentaro Kat OHiroomi AkashiTaisuke HorimotoGraduate School of Agricultural and Life Sciences The University of TokyoMahidol UniversityObihiro University of Agriculture and Veterinary Medicine2018-11-092018-11-092014-01-01Journal of Veterinary Medical Science. Vol.76, No.11 (2014), 1471-147813477439091672502-s2.0-84913582072https://repository.li.mahidol.ac.th/handle/20.500.14594/34964©2014 The Japanese Society of Veterinary Science. The entry mechanisms of Akabane virus (AKAV), Bunyaviridae family, have not yet been determined. In this study, chemical inhibitors were used to analyze endocytic mechanisms during AKAV infection of mammalian cell lines. The analyses using drug treatments followed by quantitative measurement of viral RNA and N protein revealed that AKAV enters non-bovine-derived cell lines (Vero, HmLu-1 and BHK cells) in a manner indicative of clathrin endocytosis. By contrast, AKAV infection in bovine-derived cell lines (LB9.K and MDBK cells) is independent of this pathway. Further analyses indicated that AKAV entry into bovine cell lines involves a non-clathrin, non-caveolae endocytic pathway that is dependent on dynamin. We conclude that although both cell types require a low pH for AKAV penetration, AKAV utilizes alternative entry pathways into mammalian cell lines.Mahidol UniversityVeterinaryArticleSCOPUS10.1292/jvms.14-0155