Charoonluk SangloungRuangrat BuddhirongawatrNitipan TantawiwattananonYaowalark SukthanaMahidol University2018-08-202018-08-202006-12-01The Southeast Asian journal of tropical medicine and public health. Vol.37 Suppl 3, (2006), 18-20012515622-s2.0-34447550597https://repository.li.mahidol.ac.th/handle/20.500.14594/23421In the present study, we describe in vivo cultivation to produce oocysts. Seven-day-old mice were orally infected with 100,000-120,000 Cryptosporidium oocysts. On day 8 post-infection, the mice were killed by ether, and the small and large intestines collected. A simple extraction procedure was used and purified using Ficoll gradient centrifugation. After purification, the oocysts were preserved in phosphate buffered saline with antibiotic at 4 degrees C before use.Mahidol UniversityMedicineArticleSCOPUS