Chatkul P.Satianrapapong W.Wachiradejkul W.Thampathomwong N.Sucharitakul T.Sukmak P.Sirilar P.Lertkiatdamrong P.Chatsiriwech J.Thanasumpunth N.Herabutya P.Arinno A.Pongkorpsakol P.Mahidol University2025-06-282025-06-282025-08-01Journal of Functional Foods Vol.131 (2025)17564646https://repository.li.mahidol.ac.th/handle/123456789/110928The main objectives of this study are to investigate the effects of sucralose on skin wound healing acceleration, UVB-induced epidermal epithelial damage, and to identify its underlying mechanisms of action. Sucralose significantly increased wound healing with an EC<inf>50</inf> of 30.47 μM, a maximal effect being observed at 100 μM. In addition, sucralose failed to induce wound healing in mitomycin C-primed HaCaT cell monolayers. Sucralose significantly increased keratinocyte proliferation and prevented UVB-induced cell death. These effects were suppressed by inhibitions of protein kinase A (PKA), AMP-activated protein kinase (AMPK), sirtuin-1 (SIRT-1), and extracellular signal-regulated kinase (ERK). Sucralose-induced AMPK phosphorylation was fully abolished by pretreatment with a PKA inhibitor. Sucralose-induced ERK phosphorylation was suppressed by inhibitors of AMPK and SIRT-1. Effect of sucralose-induced SIRT-1 activity was attenuated by PKA and AMPK inhibitor. Sucralose promotes wound healing and reduces keratinocyte death after UVB exposure by increasing cell proliferation via PKA/AMPK/SIRT-1/ERK-dependent mechanisms.NursingAgricultural and Biological SciencesMedicineArticleSCOPUS10.1016/j.jff.2025.1069442-s2.0-105008441788