Jiradechbadee T.Sawangjaroen N.Ranong G.N.Sila T.Buncherd H.Tun A.W.Thanapongpichat S.Mahidol University2023-06-222023-06-222022-06-01BioTechniques Vol.73 No.1 (2022) , 19-2407366205https://repository.li.mahidol.ac.th/handle/20.500.14594/87491Highly sensitive diagnostic tools are crucial for individual screening during an epidemic of leptospirosis. To aid in developing a diagnostic tool for the sensitive detection of pathogenic strains, a new approach targeting nucleic acid amplification that combines quantitative PCR (qPCR) and strand displacement isothermal amplification was evaluated. The effectiveness of the combined approach, a quantitative polymerase chain displacement reaction (qPCDR), was compared with a qPCR technique. The results showed that qPCDR presented higher sensitivity (at least tenfold) and shorter reaction time than the qPCR approach for pathogenic Leptospira spp. detection. Thus, the qPCDR-based technique developed in this study is a promising approach for pathogenic Leptospira spp. detection and the further development of a diagnostic kit.Biochemistry, Genetics and Molecular BiologyArticleSCOPUS10.2144/btn-2021-01242-s2.0-851339286281940981835698846