Artita SrimaneeJakob RegbergMattias HallbrinkKaido KurrikoffKadi Liis VeimanOpa VajraguptaÜlo LangelMahidol UniversityStockholms universitetUniversity of Tartu2018-11-092018-11-092014-01-01International Journal of Peptide Research and Therapeutics. Vol.20, No.2 (2014), 169-17815733904157331492-s2.0-84899483274https://repository.li.mahidol.ac.th/handle/20.500.14594/33458Delivery of pharmaceutical agents across a blood-brain barrier (BBB) is a challenge for brain cancer therapy. In this study, an in vitro BBB model was utilized to study the delivery of oligonucleotides across brain endothelial cells targeting to glioma cells in a Transwell™ setup. A series of novel peptides were synthesized by covalent conjugation of cell-penetrating peptides with targeting peptides for delivery of gene-based therapeutics. These peptides were screened for passage across the Transwell™ and we found the most efficient peptide PepFect32 from originating PepFect 14 coupled with the targeting peptide angiopep-2. PepFect32/pDNA nanocomplexes exhibited high transcytosis across the BBB in vitro model and the highest transfection efficiency to glioma cells. In conclusion, PepFect32 revealed the most efficient peptide-based vector for pDNA delivery across in vitro BBB model. © 2013 Springer Science+Business Media.Mahidol UniversityBiochemistry, Genetics and Molecular BiologyChemical EngineeringChemistryPharmacology, Toxicology and PharmaceuticsArticleSCOPUS10.1007/s10989-013-9378-4