Kitisin T.Muangkaew W.Thitipramote N.Pudgerd A.Sukphopetch P.Mahidol University2023-11-142023-11-142023-12-01Scientific Reports Vol.13 No.1 (2023)https://repository.li.mahidol.ac.th/handle/20.500.14594/91037Tryptophol (TOH), a fungal quorum-sensing molecule, that possesses anti-fungal activities for controlling the growth of human pathogenic fungi. In the present study, we developed TOH-containing emulgel formulations and examined the antifungal activities and potential use as topical treatments on the skin. The results showed that TOH-containing emulgel at 1000 μM has excellent physical characteristics as homogenous, stability, and inhibits the growth of 30 species of human pathogenic fungi in vitro. TOH-containing emulgel did not cause skin irritation in mouse model of irritation and in healthy human volunteers. Moreover, an increase in skin hydration and a decrease in trans-epidermal water loss (TEWL) were observed after TOH-containing emulgel treatment on human skin. Our findings indicated that TOH-containing emulgel can be utilize as an antifungal agent for topical treatment against fungal infections on the skin.MultidisciplinaryArticleSCOPUS10.1038/s41598-023-46121-z2-s2.0-8517569163520452322