Tongtavuch AnukarahanontaChutima ChudhabuddhiMahidol University2018-10-122018-10-121983-01-01Journal of Chromatography B: Biomedical Sciences and Applications. Vol.275, No.C (1983), 387-393037843472-s2.0-0020619999https://repository.li.mahidol.ac.th/handle/20.500.14594/30464A method was developed for the extraction and determination of unconjugated aflatoxins in human urine by high-performance liquid chromatography. The analysis is based on the elimination of lipid-soluble constituents other than unconjugated aflatoxins in urine by light petroleum extraction. The unconjugated aflatoxins were subsequently extracted from the aqueous phase with chloroform-acetone. Chromatography was performed isocratically with a silica column at 40°C. The resolved aflatoxins were detected and identified by ultraviolet and fluorometric detectors. The recoveries of aflatoxins B1and G1added prior to the extraction were 72% and 83%, respectively. This procedure is simple, sensitive and practically useful for epidemiological survey of unconjugated aflatoxins in human urine from areas with a high risk of aflatoxin consumption. © 1983.Mahidol UniversityChemistryArticleSCOPUS10.1016/S0378-4347(00)84384-4