S. TeerasongM. SaniP. NumsawatR. MartchooA. ChompoosorD. NacaprichaFlow Innovation-Research for Science and Technology Laboratories (FIRST Labs.King Mongkut's Institute of Technology LadkrabangKhon Kaen UniversityMahidol University2018-11-232018-11-232015-01-01Journal of Experimental Nanoscience. Vol.10, No.17 (2015), 1327-133517458099174580802-s2.0-84942197321https://repository.li.mahidol.ac.th/handle/20.500.14594/35722© 2015 Taylor & Francis. In this work, a silver nanoparticle (AgNP) coated glass slide was developed as a device for sensing hydrogen peroxide. AgNPs were synthesised using borohydride reduction with a citrate stabiliser, resulting in a negatively charged stabilised particle surface. The particles were attached to the glass surface using the layer-by-layer (LbL) technique. Poly (diallyldimethylammonium chloride) and poly (styrene sulphonate) were used as cationic and anionic polyelectrolyte layers, respectively. The glass slide was modified with polyelectrolytes leaving a cationic layer on the top surface. The AgNPs were subsequently deposited on the slide via electrostatic interaction. As a result, a dark yellow film of AgNPs was obtained with maximum absorption at 410 nm. Film fabrication based on LbL assembly provided acceptable reproducibility (relative standard deviation = 6.5%). The fabricated film had long-term stability (>6 weeks). A very small quantity of AgNPs was used in this method. Fabrication was performed under ambient conditions. Therefore this fabrication was considered as a green method. The AgNP modified slide was developed to sense hydrogen peroxide. Detection is based upon oxidation of AgNPs by hydrogen peroxide. This results in a change in colour of the film from dark yellow to colourless. Linear calibration was obtained over the range of 1.0--100.0 mM of hydrogen peroxide. The device was successfully used for measuring hydrogen peroxide in urine.Mahidol UniversityChemical EngineeringEngineeringMaterials ScienceArticleSCOPUS10.1080/17458080.2015.1007097