Santi ManeewatchJeeraphong ThanongsaksrikulThaweesak SongsermKanyarat Thueng-InKasem KulkeawUmaporn ThathaisongPotjanee SrimanotePongsri TongtawePramuan TapchaisriWanpen ChaicumpaMahidol UniversityKasetsart UniversityThammasat University2018-09-132018-09-132009-06-29Antiviral Therapy. Vol.14, No.2 (2009), 221-230135965352-s2.0-67149113906https://repository.li.mahidol.ac.th/handle/20.500.14594/28041Background: Human antibodies that interfere with the biological activity of haemagglutinins (HAs) of influenza viruses have high potential as an antiviral agent. Methods: Human single-chain antibody fragments (HuScFv) to recombinant and native HAs of the influenza virus H5N1 subtype were produced using a human antibody phage display library with the intention to increase the therapeutic arsenal against this highly pathogenic virus. Results: The HuScFv inhibited HA activity and neutralized infectivity of both homologous and heterologous strains and clades of the H5N1 subtype in Madin-Darby canine kidney cell cultures. Intraperitoneally injected HuScFv also mediated immunotherapeutic protection in mice that were intranasally challenged with highly pathogenic H5N1 viruses belonging to different strains and clades. Conclusions: Our data indicate that it might be worth pursuing these HuScFv further for future consideration as candidates for influenza intervention and treatment. © 2009 International Medical Press.Mahidol UniversityMedicinePharmacology, Toxicology and PharmaceuticsArticleSCOPUS