Prasan TangyuenyongwatanaVichien KeeratinijakalWandee GritsanapanRangsit UniversityKasetsart UniversityMahidol University2018-06-112018-06-112012-11-01Journal of AOAC International. Vol.95, No.6 (2012), 1614-1617106032712-s2.0-84871338584https://repository.li.mahidol.ac.th/handle/20.500.14594/13379Zingiber cassumunar (Zingiberaceae) has been widely used as a traditional medicine in southeast Asia, especially Thailand, for treatment of asthma, inflammation, and joint pain. (E)-4-(3,4- dimethoxyphenyl)butadiene, or DMPBD, a major component in the rhizome, has been reported to be an active anti-inflammatory agent. This research developed a TLC-densitometry method for the simultaneous quantification of DMPBD in the rhizome extracts of four varieties of Z. cassumunar. DMPBD was found in the range of 1.80 ± 0.01 to 3.23 ± 0.10% (w/w). The method was validated for linearity, precision, accuracy, robustness, LOD, and LOQ. The linearity was indicated by a good regression coefficient 0.9940 and covered the range of 130 to 703 ng/band. Intraday and interday precision (RSD) were less than 2%. Accuracy of the method was evaluated by a recovery study conducted at three different levels; the average recovery was 103.1%. The LOD and LOQ were 10 and 40 ng, respectively. The proposed TLC-densitometry method was simple, precise, specific, sensitive, and inexpensive. This method can be used for routine QC of raw materials of Z. cassumunar rhizomes, extracts, and its products, especially Prasaplai, a Thai traditional medicine for relieving dysmenorrhea.Mahidol UniversityAgricultural and Biological SciencesChemistryEnvironmental SciencePharmacology, Toxicology and PharmaceuticsArticleSCOPUS10.5740/jaoacint.11-188