Teeraporn SuteewongJitrada WongpreechaDuangporn PolpanichKulachart JangpatarapongsaChariya KaewsanehaPramuan TangboriboonratKing Mongkut's Institute of Technology LadkrabangThailand National Nanotechnology CenterMahidol UniversitySirindhorn International Institute of Technology, Thammasat University2020-01-272020-01-272019-02-01Colloids and Surfaces B: Biointerfaces. Vol.174, (2019), 544-55218734367092777652-s2.0-85057260028https://repository.li.mahidol.ac.th/handle/20.500.14594/50277© 2018 Elsevier B.V. The antibacterial activity in sulphur prevulcanized natural rubber (SPNR) latex film was effectively improved by deposition of poly(methyl methacrylate) (PMMA) particles encircled with chitosan-coated silver nanoparticles (AgNPs-CS). With the focus on a green process, CS was selected as a safe reducing and stabilizing agent for the one-step synthesis of AgNPs-CS (38 nm, +40.4 mV) in an autoclave. The adsorption of small-sized AgNPs-CS directly onto rubber film did not provide an inhibitory effect on S. aureus. It also had a low antibacterial effect on E. coli. This is because of the particles becoming completely/partially submerged into the soft rubber matrix upon drying. Hence, the AgNPs-CS were fabricated as a shell surrounding a rigid PMMA core (496 nm, -30.9 mV). This was done using a heterocoagulation technique prior to coating on SPNR film. The presence of PMMA/AgNPs-CS on the surface of SPNR film effectively increased the surface roughness from ca. 44 to 150 nm. This substantially promoted the antibacterial activity against E. coli and S. aureus by way of contact killing and repelling mechanisms. The cytotoxicity on L-929 fibroblasts was also suppressed. This study would be, therefore, applicable to the development of antibacterial SPNR film with high surface roughness, low cytotoxicity. It could also be applied for other soft substrates.Mahidol UniversityBiochemistry, Genetics and Molecular BiologyChemical EngineeringChemistryArticleSCOPUS10.1016/j.colsurfb.2018.11.037