Muhammad Hatta PrabowoSupawat ChatchenPatsamon RijiravanichKriengsak LimkittikulWerasak SurareungchaiUniversitas Islam IndonesiaMahidol UniversityKing Mongkut s University of Technology Thonburi2020-03-262020-03-262020-01-01Analytical and Bioanalytical Chemistry. (2020)16182650161826422-s2.0-85081898026https://repository.li.mahidol.ac.th/handle/123456789/53609© 2020, Springer-Verlag GmbH Germany, part of Springer Nature. The diagnosis of dengue infection is still a critical factor determining success in the clinical management and treatment of patients. Here, the development of microfluidic paper-based analytical devices (μPADs) utilizing a sandwich immunoassay on wax patterned paper functionalized with anti-dengue NS1 monoclonal antibodies for point-of-care detection of dengue NS1 (DEN-NS1-PAD) is reported. Various assay conditions, including the length of the channel and diluent, were optimized, and the response detected by the naked eye and digitized images within 20–30 min. The DEN-NS1-PAD was successfully tested in the field for detecting dengue NS1 in buffer, cell culture media, and human serum. The limit of detection (LoD) of the DEN-NS1-PAD obtained with the naked eye, scanner, and a smartphone camera was 200, 46.7, and 74.8 ng mL−1, respectively. The repeatability, reproducibility, and stability of the DEN-NS1-PAD were also evaluated. High true specificity and sensitivity in the serum of pediatric patients were observed. These evaluation results confirm that the DEN-NS1-PAD can potentially be used in point-of-care dengue diagnostics, which can significantly impact on the spreading of mosquito-borne diseases, which are likely to become more prevalent with the effects of global warming. [Figure not available: see fulltext.].Mahidol UniversityBiochemistry, Genetics and Molecular BiologyChemistryArticleSCOPUS10.1007/s00216-020-02527-6