Porncharn SaitongdeeAndrzej LoeschGillian KnightPamela MilnerGeoffrey BurnstockUCLMahidol University2018-09-072018-09-071999-01-01Endothelium: Journal of Endothelial Cell Research. Vol.6, No.3 (1999), 197-207106233292-s2.0-0032893614https://repository.li.mahidol.ac.th/handle/20.500.14594/25360This is a study of the electron-immunocytochemical localization of nitric oxide synthase (type III) and endothelin in renal and mesenteric artery endothelial cells of normal (active) and hibernating hamsters, as well as hamsters exposed to the cold but not hibernating, and hamsters aroused for 2 h following hibernation. In the renal artery of hibernating hamsters and cold-exposed hamsters, a subpopulation of nitric oxide synthase-positive endothelial cells displayed immunoprecipitate predominantly in the vicinity of the Golgi complex indicating intracellular translocation from the cytoplasm to the Golgi complex. In hibernating animals, the percentages of both nitric oxide synthase-positive and endothelin-positive endothelial cella were notably lower than those observed either in active, cold-exposed or aroused animals. These changes may reflect a reduced endothelial contribution to the maintenance of vascular tone in these vessels during hibernation and an upregulation of expression of nitric oxide synthase and endothelin in the endothelium early on during arousal from hibernation.Mahidol UniversityBiochemistry, Genetics and Molecular BiologyArticleSCOPUS10.3109/10623329909053410