Rachaneepas TungkanakStitaya SirisinhaMahidol UniversityMahidol University2018-06-012018-06-011978-01-01Proceedings of the Society for Experimental Biology and Medicine. Vol.159, No.2 (1978), 176-17915353699003797272-s2.0-0018248915https://repository.li.mahidol.ac.th/handle/20.500.14594/13046The anti-idiotypic response of BALB/c mice to myeloma protein of BALB/c origin (purified Protein-315 from a plasmacytoma MOPC-315) was analyzed for its antigen-binding capacity and susceptibility to inhibition by excess hapten (DNP-lysine). The results showed that the anti-idiotypic antibody that is sensitive to inhibition by hapten could be detected for at least 3 months after completion of the primary course of immunization. Following a single booster injection, there was an increase of the antigen-binding capacity and the susceptibility of these post-boosting antisera to inhibition by hapten was markedly reduced (from more than 80% to less than 10% under the assay system employed). However, the hapten inhibition value gradually returned toward the preboosting level within a few weeks. The data obtained suggest that the change in the hapten inhibition value after boosting is associated with increased affinity rather than a shift in specificity. © 1978, SAGE Publications. All rights reserved.Mahidol UniversityBiochemistry, Genetics and Molecular BiologyArticleSCOPUS10.3181/00379727-159-40308