Chanchao LorthongpanichNittaya JiamvoraphongPrapasri SupakunNattaya DamkhamPapussorn TerbtoSupaporn WaeteekulYaowalak U-pratyaChuti LaowtammathronSurapol IssaragrisilFaculty of Medicine, Siriraj Hospital, Mahidol UniversityWattanosoth Hospital2020-01-272020-01-272019-12-01Stem Cell Research. Vol.41, (2019)18767753187350612-s2.0-85074105106https://repository.li.mahidol.ac.th/handle/20.500.14594/50007© 2019 The Author(s) WWTR1 or TAZ (WWTR1/TAZ) is a transcriptional coactivator that acts as a downstream regulatory target in the Hippo signaling pathway, which plays a pivotal role in regulating cell proliferation and anti-apoptosis. It has been shown in other cell types that WWTR1/TAZ plays a redundant role to its homolog YAP1. Using CRISPR/Cas9 gene editing, we established the WWTR1/TAZ-KO cell line, which features homozygous deletion of WWTR1 gene from human iPSCs. The established WWTR1/YAZ-KO cell line maintained the pluripotent phenotype, the ability to differentiate into all three embryonic germ layers, and normal karyotype.Mahidol UniversityBiochemistry, Genetics and Molecular BiologyArticleSCOPUS10.1016/j.scr.2019.101634