Aurachorn InkanuwatRomteera SukaboonOnrapak ReamtongPravit AsawanondaAke PattaratanakunTanatorn SaisavoeyPapassara SangtanooAphichart KarnchanatatChulalongkorn UniversityMahidol University2020-01-272020-01-272019-04-01Food Technology and Biotechnology. Vol.57, No.2 (2019), 200-21113342606133098622-s2.0-85069807751https://repository.li.mahidol.ac.th/handle/20.500.14594/49790© 2019 University of Zagreb. Nitric oxide (NO) plays a key role in the pathogenesis of inflammation and has been implicated in endotoxin-induced tissue injury. Chicken feather meal is a rich source of amino acids that may serve as a peptide hydrolysate to inhibit NO activity. Anti-inflammatory hydrolysates of chicken feather meal were prepared and fractionated into five samples based on molecular mass. The smallest fraction (<0.65 kDa) exhibited the highest NO inhibitory activity without cytotoxicity towards macrophage RAW 264.7 cells. Further subfractions were sufficient to obtain amino acid sequences by Q-TOF LC-MS/MS ESI analysis. Of these, the SNPSVAGVR (885.97 Da) peptide and its corresponding pure synthetic peptide have inhibitory activity against NO production by RAW 264.7 cells (IC50=(55.2±0.2) mM) without cytotoxicity. Reverse transcriptase polymerase chain reaction (RT-PCR) and quantitative real-time RT-PCR results revealed that the peptide of the obtained fraction reduced transcript expression levels of the pro-inflammatory cytokines iNOS, TNF-a, COX-2 and IL-6 in lipopolysaccharide-stimulated RAW 264.7 cells. These results suggest that the peptides derived from the chicken feather meal protein could potentially be used as a promising ingredient in functional foods or nutraceuticals against inflammatory diseases.Mahidol UniversityAgricultural and Biological SciencesBiochemistry, Genetics and Molecular BiologyChemical EngineeringEngineeringArticleSCOPUS10.17113/ftb.57.02.19.5964