Noppawong S.Pratabsingha J.Thamsoonthorn C.Vichathai W.Saikaew P.Mahidol University2025-12-072025-12-072022-01-01Journal of Adhesive Dentistry Vol.24 No.1 (2022) , 421-42614615185https://repository.li.mahidol.ac.th/handle/123456789/113416Purpose: To evaluate the microtensile bond strength (μTBS) of three universal adhesives to dentin contaminated with a hemostatic agent. Materials and Methods: Ninety-six human premolars were cut to expose mid-coronal dentin. The teeth were randomly divided into a control group (uncontaminated dentin) and an experimental group in which a hemostatic agent was applied (contaminated dentin). Each group was further divided into 6 subgroups according to universal adhesives – SBU (Single Bond Universal), OPU (OptiBond Universal), CBQ (Clearfil Universal Bond Quick) – and etching mode, either etch- and-rinse (ER) or self-etch (SE). Following adhesive application, composite was placed in two increments and light cured. The specimens were stored in distilled water at 37°C for 24 h. The μTBS test was performed using a universal testing machine. Failure mode was assessed using a light microscope. The data were statistically analyzed using three-way ANOVA followed by Scheffe’s test (p < 0.05). The resin-dentin interface was observed using scanning electron microscopy. Results: Significantly lower bond strength was observed when the universal adhesives were bonded to contaminated dentin in SE mode (p < 0.05). In contrast, the μTBS of the universal adhesives in ER mode was not affected by contamination (p > 0.05). The μTBS of CBQ to contaminated dentin was significantly lower than that of the other adhesives. Observation of the resin-dentin interface revealed limited resin penetration when the universal adhesive was applied in SE mode on contaminated dentin. Conclusion: Contaminating the dentin with a hemostatic agent significantly reduced the μTBS of the universal adhesives in SE mode. However, this adverse effect was not found when the universal adhesives were used in ER mode.DentistryArticleSCOPUS10.3290/j.jad.b36017692-s2.0-851429396771757998836416598