Arian R.Behzadi Far M.Sadeghi R.Farnaghi F.Safizadeh M.Subramanian P.S.Miller N.R.Suwan Y.Kajornrojanaruk P.Kafieh R.Aghsaei Fard M.Mahidol University2026-04-182026-04-182026-04-06Translational Vision Science Technology Vol.15 No.4 (2026) , 7https://repository.li.mahidol.ac.th/handle/123456789/116249Purpose: To determine the ability of a deep learning (DL) algorithm to segment retinal nerve fiber layer (RNFL) from optical coherence tomography (OCT) scans in glaucomatous optic neuropathies and anterior optic neuropathies with optic disc edema. Methods: RNFL-Net was developed after preprocessing and automatically removing blood vessels from peripapillary OCT B-scans. It was trained and validated on 1065 RNFL OCT B-scans, and its performance was assessed using 265 test scans. Two different datasets were used for external testing. Results: The study involved 106 eyes from healthy controls, 118 eyes with optic disc edema, and 60 eyes with glaucoma for training and validation. The segmentation method achieved a Dice coefficient of 0.95 for the validation dataset and 0.92 for test images when compared with manual segmentation. In measuring RNFL thickness in glaucoma-affected eyes, RNFL-Net showed a mean absolute error (MAE) of 6.21 µm and a mean absolute percentage error (MAPE) of 11.24%. The standard OCT device had MAE of 11.05 µm and MAPE of 16.8%. For optic disc edema, the RNFL-Net MAE was 13.04 µm and MAPE 5.71%, whereas the OCT device reported MAE of 22.94 µm and MAPE of 11.2%. For the external validation data, MAE values for glaucoma (n = 157) and disc edema (n = 32) cases were 7.19 ± 0.14 and 15.41 ± 0.32, respectively. Conclusions: RNFL-Net can accurately segment RNFL, whereas standard OCT devices produce lower measurements, especially in disc edema. Translational Relevance: RNFL thickness measurements from RNFL-Net matched the ground truth in glaucoma and optic disc edema cases.MedicineEngineeringArticleSCOPUS10.1167/tvst.15.4.72-s2.0-1050354712932164259141960956