S. SoontornyataraP. KlammornMahidol University2021-02-032021-02-032020-12-08Acta Horticulturae. Vol.1298, (2020), 485-48924066168056775722-s2.0-85099512725https://repository.li.mahidol.ac.th/handle/20.500.14594/60842© 2020 International Society for Horticultural Science. All rights reserved. Wan Khanmak (Aglaonema simplex (Blume) Blume) is a kind of Thai herb. The ripe fruits are extensively used as longevity enhancer and anti-asthma medication, but the number of fruits plant-1was only 3-14. This research project aims to find the most appropriate tissue culture protocol for mass propagation of Wan Khanmak herb. The experimental design was completely randomized design (CRD). The axillary buds of Wan Khanmak were incubated on MS medium supplemented with 1-naphthaleneacetic acid (NAA) and thidiazuron (TDZ) at various doses; 0:0, 0.25:0.5, 0.25:1.0, 0.25:1.5, 0.25:2.0 and 0.25:2.5 mg L-1. The results showed that the most effective medium was MS medium supplemented with NAA and TDZ at 0.25:2.5 mg L-1ratio which produced the highest number of average shoots per culture (4.49 shoots) with an average shoots length 1.60 cm in 8 weeks of culture. This treatment gave the fastest time to produce the first shoot in 8.5 days.Mahidol UniversityAgricultural and Biological SciencesArts and HumanitiesConference PaperSCOPUS10.17660/ActaHortic.2020.1298.66