Shinichi KakudaShigeaki AbePipop SaikaewAlmas Afm ChowdhuryHidehiko SanoHokkaido UniversityMahidol UniversitySapporo Dental College and Hospital2019-08-232019-08-232018-01-01Nano Biomedicine. Vol.10, No.2 (2018), 48-5221854734188351982-s2.0-85067191687https://repository.li.mahidol.ac.th/handle/20.500.14594/45849© 2018 National Institute of Informatics. All rights reserved. Glass-ionomer cement is primarily used for its ability to prevent caries due to sustained release of fluoride. In recent years, it has been proven as a carrier of various kinds of ions other than fluoride, and attention is paid to its property as a bioactive material. However, the release of ions from the material is not stable, and therefore, the reproducibility of the experiments is poor and variations are common. In this study, we investigated whether the presence or absence of stirring affects the stability and reproducibility of the reactions, aiming to optimize the research conditions for obtaining more consistent results. Our results suggest that the values obtained with continuous stirring are significantly more stable than the values obtained from stationary conditions.Mahidol UniversityEngineeringArticleSCOPUS10.11344/nano.10.48