Weeraya ThongkumUmpa YasamutKoollawat ChupraditSupachai SakkhachornphopJiraprapa WipasaKanokporn SornsuwanOn Anong JuntitRawiwan PornprasitWanwisa ThongkamwitoonJirapan ChaichananJaruwan KhaoplabChonnikarn ChanpradabWatchara KasinrerkChatchai TayapiwatanaMahidol UniversityChiang Mai University2022-08-042022-08-042021-06-01Diagnostics. Vol.11, No.6 (2021)207544182-s2.0-85107827988https://repository.li.mahidol.ac.th/handle/20.500.14594/76156Adult-onset immunodeficiency syndrome (AOID) patients with autoantibodies (autoAbs) against interferon-gamma (IFN-γ) generally suffer from recurrent and recalcitrant disseminated non-tuberculous mycobacterial diseases. Since the early stages of AOID do not present specific symptoms, diagnosis and treatment of the condition are not practical. A simplified diagnostic method for differentiating AOID from other immunodeficiencies, such as HIV infection, was cre-ated. Anti-IFN-γ is generally identified using enzyme-linked immunosorbent assay (ELISA), which involves an instrument and a cumbersome process. Recombinant IFN-γ indirectly conjugated to colloidal gold was used in the modified immunochromatographic (IC) strips. The biotinylated-IFN-γ was incorporated with colloidal-gold-labeled 6HIS-maltose binding protein-monomeric streptav-idin (6HISMBP-mSA) and absorbed at the conjugate pad. The efficacy of the IC strip upon applying an anti-IFN-γ autoAb cut-off ELISA titer of 2500, the sensitivity and specificity were 84% and 90.24%, respectively. When a cut-off ELISA titer of 500 was applied, the sensitivity and specificity were 73.52% and 100%, respectively.Mahidol UniversityBiochemistry, Genetics and Molecular BiologyArticleSCOPUS10.3390/diagnostics11060987