Montri RatanajanchaiDon Haeng LeePanya SunintaboonSu Geun YangUtah-Inha DDS and Advanced TherapeuticsMahidol UniversityInha University, College of Medicine2018-11-092018-11-092014-02-01Journal of Colloid and Interface Science. Vol.415, (2014), 70-7610957103002197972-s2.0-84887335684https://repository.li.mahidol.ac.th/handle/123456789/33575Herein, we introduced amine-functionalized core-shell nanoparticles (Polymethyl methacrylate/Polyethyleneimine; PMMA/PEI) with surface primary amines (3.15×105groups/particle) and uniform size distribution (150-200nm) that were prepared by one-step photo-induced emulsion polymerization. Further PEI-surface was modified with diethylenetriamine pentaacetic acid (DTPA) and introduced with Gd(III). The modified particles possessing DTPA can entrap a high content of Gd(III) ions of over 5.5×104Gd/particle with stable chelation (no release of free Gd) at least 7h. The Gd-DTPA-conjugated core-shell nanoparticles (PMMA/PEI-DTPA-Gd NPs) enhanced the MRI intensity more than Primovist (a commercial hepatic contrast agent). Moreover, the PMMA/PEI-DTPA-Gd NPs showed non-cytotoxicity up to 250μM in normal liver cells. Thus, in vitro data suggested the PMMA/PEI-DTPA-Gd NPs is promising delivery system as a superior MRI contrast agent, especially for hepatic lesion targeted MR imaging. © 2013 Elsevier Inc.Mahidol UniversityChemical EngineeringMaterials ScienceArticleSCOPUS10.1016/j.jcis.2013.09.049