Generation of a human induced pluripotent stem cell line (MUi010-A) from skin fibroblast of patient carrying a c.2104C>T mutation in MYH9 gene

Amornrat TangprasittipapBunyada JittorntrumPraguywan KadegasemWerasak SasanakulNongnuch SirachainanNarisorn KitiyanantSuradej HongengFaculty of Medicine, Ramathibodi Hospital, Mahidol UniversityMahidol University2020-01-272020-01-272019-04-01Stem Cell Research. Vol.36, (2019)18767753187350612-s2.0-85061823358https://repository.li.mahidol.ac.th/handle/20.500.14594/50207© 2019 Mutations in MYH9 gene is one of the major causes of inherited thrombocytopenia resulted from nonfunctional myosin-9 protein. We have generated a human induced pluripotent stem cell line MUi010-A from skin fibroblasts of a patient who had a point mutation c.2104C>T (p.R702C) in the exon 16 of MYH9 gene using a non-integrative reprogramming method. The MUi010-A exhibited embryonic stem cell-like characteristics with consistent pluripotent markers expression, was capable of all three embryonic germ layers differentiation, and had a normal karyotype.Mahidol UniversityBiochemistry, Genetics and Molecular BiologyGeneration of a human induced pluripotent stem cell line (MUi010-A) from skin fibroblast of patient carrying a c.2104C>T mutation in MYH9 geneArticleSCOPUS10.1016/j.scr.2019.101397