Paron Dekumyoyพารณ ดีคำย้อยSupaporn Nuamtanongสุภาพร น่วมทะนงSurapol Sa-nguankiatสุรพล สงวนเกียรติDuangrudee Chindanondดวงฤดี จินดานนท์Mahidol University. Faculty of Tropical Medicine. Department of HelminthologyMahidol University. Faculty of Tropical Medicine. The Hospital for Tropical Diseases2016-02-022021-09-022016-02-022021-09-022016-02-022001https://repository.li.mahidol.ac.th/handle/20.500.14594/63404Joint International Tropical Medicine Meeting 2001: Century Pard Hotel, Bangkok, Thailand 8-10 August 2001: abstract. Bangkok: Faculty of Tropical Medicine, Mahidol University; 2001. p.206Two preparations of antigens for diagnosis of strongyloidiasis were prepared by the extract of infective larvae of Strongyloides stercoralis designed as crude antigen (CA) and molecular weight cut-off-off antigen (MWCOA). Both antigens were analysede by indirect ELISA against sera of strongyloidiasis (26 cases), other helminthiases (167) and normal controls (30). The larvae were obtained from fecal culture by some modification of the polyethylene tube technique after screening tests by simple smear technique had been done, in triples per case. The larvae were extracted with distilled water and further sonicated to obtain the supernatant, CA. A part of the CA was separated for an antigen containing molecules lower than 30 kDa by ultrafree-MC centrifugal filter tube (PLTK), designed as MWCOA. On analysis of the tests, CA gave 96.15% sensivity and 40.12% (67-176) specificity at cut-off value 0.980 (5SD); meanwhile, the false positives were 19 from 20 different kinds of helminthiases. Only paragonimiasis westermani infections showed true negative. The MWCOA showed 96.15% sensitivity at cut-off value 0.71 (4SD) and the specificity of the test was 79.04% (132/167). Higher than that of CA. False positives also appeared with 15 kind of helminthiases , but no cross reaction occurred with capillariasis, taeniasis, paragonimiasis heterotremus, paragonimiasis westermani or schistosomiasis, This study produced greater specificity for MWCOA than that of CA. Due to the reduction of cross-reactivity, a purification of MWCOA will be done to gain particularly antigenic molecules for strongyloidiasis diagnosisengMahidol UniversityStrongyloidiasisProceeding Poster