Adeline C.Y. ChuaJessica Jie Ying OngBenoit MalleretRossarin SuwanaruskVarakorn KosaisaveeAnne Marie ZeemanCaitlin A. CooperKevin S.W. TanRou ZhangBee Huat TanSiti Nurdiana AbasAndy YipAnne ElliotChester J. JoynerJee Sun ChoKate BreyerSzczepan BaranAmber LangeSteven P. MaherFrançois NostenChristophe BodenreiderBryan K.S. YeungDominique MazierMary R. GalinskiNathalie Dereuddre-BosquetRoger Le GrandClemens H.M. KockenLaurent RéniaDennis E. KyleThierry T. DiaganaGeorges SnounouBruce RussellPablo BifaniA-Star, Singapore Immunology NetworkCentre de Recherche en Immunologie des Infections Virales et des Maladies Auto-ImmunesLondon School of Hygiene & Tropical MedicineThe University of GeorgiaYong Loo Lin School of MedicineNovartis Institute for Tropical Diseases Pte. Ltd.Biomedical Primate Research Centre - RijswijkUniversity of OtagoMahidol UniversityNuffield Department of Clinical MedicineSorbonne UniversiteEmory UniversityInsermNovartis Institutes for BioMedical Research2020-01-272020-01-272019-12-01Nature Communications. Vol.10, No.1 (2019)204117232-s2.0-85070687044https://repository.li.mahidol.ac.th/handle/20.500.14594/50025© 2019, The Author(s). The ability to culture pathogenic organisms substantially enhances the quest for fundamental knowledge and the development of vaccines and drugs. Thus, the elaboration of a protocol for the in vitro cultivation of the erythrocytic stages of Plasmodium falciparum revolutionized research on this important parasite. However, for P. vivax, the most widely distributed and difficult to treat malaria parasite, a strict preference for reticulocytes thwarts efforts to maintain it in vitro. Cultivation of P. cynomolgi, a macaque-infecting species phylogenetically close to P. vivax, was briefly reported in the early 1980s, but not pursued further. Here, we define the conditions under which P. cynomolgi can be adapted to long term in vitro culture to yield parasites that share many of the morphological and phenotypic features of P. vivax. We further validate the potential of this culture system for high-throughput screening to prime and accelerate anti-P. vivax drug discovery efforts.Mahidol UniversityBiochemistry, Genetics and Molecular BiologyChemistryArticleSCOPUS10.1038/s41467-019-11332-4