Kanda EkcharoenkulPopchai NgamskulrungrojKeerati JoyjamrasAmornrut LeelapornAzian HarunPattarachai KiratisinFaculty of Medicine, Siriraj Hospital, Mahidol UniversitySchool of Medical Sciences - Universiti Sains Malaysia2019-08-282019-08-282018-03-01Siriraj Medical Journal. Vol.70, No.2 (2018), 127-130222880822-s2.0-85051599308https://repository.li.mahidol.ac.th/handle/20.500.14594/46901© 2018, Faculty of Medicine Siriraj Hospital, Mahidol University. Objective: Matrix Assisted Laser Desorption Ionization Time-Of-Flight mass spectrometry (MALDI-TOF MS) is a rapid and cost-effective technique that serves as an alternative method for bacterial identification in microbiology laboratories. This study aimed to determine the performance of MALDI-TOF MS for identifying uncommon and/ or fastidious bacteria comparing with automated identification method. Methods: We observed the performances of original and extended databases after adding of in-house MALDI-TOF MS database for a total of 54 isolates belonging to 16 genera of uncommon and/or fastidious bacteria. Results: The extended database improved identification of these bacteria significantly except for Listeria monocytogenes and Elizabethkingia meningoseptica. Conclusion: This study provides a possibility for identification of uncommon and/or fastidious bacteria by MALDITOF MS method.Mahidol UniversityMedicineArticleSCOPUS10.14456/smj.2018.21