Impaired interaction of α-haemoglobin-stabilising protein with α-globin termination mutant in a yeast two-hybrid system

Chairat TurbpaiboonThawornchai LimjindapornWiyada WongwiwatYaowalak U-PratyaNoppadol SiritanaratkulPa Thai YenchitsomanusSarawut JitrapakdeePrapon WilairatMahidol University2018-08-202018-08-202006-02-01British Journal of Haematology. Vol.132, No.3 (2006), 370-37313652141000710482-s2.0-33644868959https://repository.li.mahidol.ac.th/handle/20.500.14594/23824α-Thalassaemia caused by α-globin gene termination codon mutations (αT-globin) has been explained by their inherent mRNA instability and by oxidative damage arising from the presence of membrane-bound αT-globin chains. To better understand the latter phenomenon, a yeast two-hybrid system was used to assay the interaction between αT-globin and its molecular chaperone, α-haemoglobin- stabilising protein (AHSP) and impaired binding of αT-globin with AHSP compared with αwild-type-globin was observed. © 2005 Blackwell Publishing Ltd.Mahidol UniversityMedicineImpaired interaction of α-haemoglobin-stabilising protein with α-globin termination mutant in a yeast two-hybrid systemArticleSCOPUS10.1111/j.1365-2141.2005.05865.x