Boontun C.Vatanyoopaisarn S.Domrongpokkaphan V.Phalakornkule C.Chinli R.Thitisak P.Hankla S.Mahidol University2024-04-182024-04-182024-04-01Applied Science and Engineering Progress Vol.17 No.2 (2024)26729156https://repository.li.mahidol.ac.th/handle/123456789/98031Beneficial microbes, such as probiotic bacteria, are increasingly in demand in the food and feed industry. Lactic acid bacteria and bifidobacteria are commonly used as commercial probiotics, only a few species have been isolated from Southeast Asia areas. This study employed criteria including antimicrobial activity, the release of digestive enzymes, and the absence of antibiotic-resistant (AMR) genes to screen potential local isolates. The results revealed that 4 out of 16 isolates met these criteria, displaying anti-foodborne bacterial activities and a lack of fifty-one tested AMR genes. Furthermore, the four selected isolates demonstrated the production of extracellular digestive enzymes, including amylase, lipase, protease, β-glucanase, and cellulase, with enzyme indices ranging from 1.09-1.31. Among these isolates, two potential probiotics were identified as Bifidobacterium animalis subsp. lactis (strain H9-01) and Lactobacillus reuteri (strain P4-S03). Importantly, both species are approved for use as food and feed supplements in accordance with Thai regulations. This research outlines an approach for screening potential probiotics for industrial-scale applications.Materials ScienceChemical EngineeringComputer ScienceEngineeringArticleSCOPUS10.14416/j.asep.2023.12.0032-s2.0-8519016108926730421