Ludowyke N.Phumiphanjarphak W.Apiwattanakul N.Manopwisedjaroen S.Pakakasama S.Sensorn I.Pasomsub E.Chantratita W.Hongeng S.Aiewsakun P.Thitithanyanont A.Mahidol University2023-06-182023-06-182022-04-01Viruses Vol.14 No.4 (2022)https://repository.li.mahidol.ac.th/handle/20.500.14594/84998Human pegivirus-1 (HPgV-1) is a lymphotropic human virus, typically considered nonpathogenic, but its infection can sometimes cause persistent viremia both in immunocompetent and immunosuppressed individuals. In a viral discovery research program in hematopoietic stem cell transplant (HSCT) pediatric patients, HPgV-1 was detected in 3 out of 14 patients (21.4%) using a target enrichment next-generation sequencing method, and the presence of the viruses was confirmed by agent-specific qRT-PCR assays. For the first time in this patient cohort, complete genomes of HPgV-1 were acquired and characterized. Phylogenetic analyses indicated that two patients had HPgV-1 genotype 2 and one had HPgV-1 genotype 3. Intra-host genomic variations were described and discussed. Our results highlight the necessity to screen HSCT patients and blood and stem cell donors to reduce the potential risk of HPgV-1 transmission.Immunology and MicrobiologyArticleSCOPUS10.3390/v140407962-s2.0-851288014691999491535458526