Genomic insights into ceftazidime resistance in Burkholderia pseudomallei: discovery of A172T mutation and palindromic GC-rich repeat sequences facilitating penA duplication and amplification

Tuanyok A.Nakajima C.Noll T.Khan M.S.R.Khrongsee P.Yowell C.A.Xiao Y.P.Wuthiekanun V.Chantratita N.Heine H.Subramaniam K.Suzuki Y.Limmathurotsakul D.Mergia A.Mahidol University2025-08-232025-08-232025-08-06Antimicrobial Agents and Chemotherapy Vol.69 No.8 (2025) , e0022025https://repository.li.mahidol.ac.th/handle/123456789/111743Ceftazidime (CAZ) resistance in Burkholderia pseudomallei, the causative agent of melioidosis, complicates treatment in endemic regions. This study identified a novel A172T mutation and other known penA mutations as critical contributors to CAZ resistance in a large Thai strain collection. Frequent gene duplication and amplification of penA, likely driven by Palindromic GC-Rich Repeat Sequences, highlights the urgent need for rapid diagnostics and optimized treatment strategies to manage this life-threatening disease effectively.Pharmacology, Toxicology and PharmaceuticsMedicineGenomic insights into ceftazidime resistance in Burkholderia pseudomallei: discovery of A172T mutation and palindromic GC-rich repeat sequences facilitating penA duplication and amplificationArticleSCOPUS10.1128/aac.00220-252-s2.0-1050131569581098659640689768