Ngamratanapaiboon S.Pornchokchai K.Wongpitoonmanachai S.Pholkla P.Srikornvit N.Mo J.Hongthawonsiri P.Yambangyang P.Akrachalanont P.Mahidol University2023-09-242023-09-242023-09-01Research in Pharmaceutical Sciences Vol.18 No.5 (2023) , 517-52717355362https://repository.li.mahidol.ac.th/handle/20.500.14594/90199Background and purpose: The use of fluoxetine raises the risk of pancreatic beta-cell dysfunction. However, the specific mechanism behind its mechanism of action in beta cells is unknown. This study investigated the cellular response of MIN6 cells to fluoxetine using untargeted cell-based metabolomics. Experimental approach: Metabolic profiling of MIN6 cells was performed using liquid chromatography-high resolution mass spectrometry (LC-HRMS) analysis on samples prepared under optimized conditions, followed by principal component analysis, partial least squares-discriminant analysis, and pair-wise orthogonal projections to latent structures discriminant analyses. Findings/Results: Sixty-six metabolites that had been differentially expressed between the control and fluoxetine-treated groups demonstrated that the citric acid cycle is mainly perturbed by fluoxetine treatment. Conclusion and implications: The current study provides insights into the molecular mechanisms of fluoxetine effects in MIN6 cells.Pharmacology, Toxicology and PharmaceuticsArticleSCOPUS10.4103/1735-5362.3837072-s2.0-8517136632617359414