Wongwarut BoonyanugomolNarong KhuntikeoAke PugkhemKookwan SawadpanichChariya HahnvajanawongPhattharaphon WongphutornBandit KhampoosaChariya ChomvarinMahidol University. Amnatchareon CampusKhon Kaen University. Faculty of Medicine. Department of MicrobiologyKhon Kaen University. Faculty of Medicine. Department of SurgeryKhon Kaen University. Faculty of Medicine. Department of MedicineKhon Kaen University. Faculty of Medicine. Liver Fluke and CholangiocarcinomaResearch Center,King Mongkut’s University of Technology Thonburi. School of Bioinformatics and Systems Biology2020-01-072020-01-072020-01-072017The Journal of Infection in Developing Countries. Vol.11, No.1 (2017), 42-50.https://repository.li.mahidol.ac.th/handle/20.500.14594/48687This is an open-access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly citedIntroduction: H. pylorihas been detected in patients with hepatobiliary diseases. It is currently unclear whether the H. pyloridetected in hepatobiliary patients are genetically similar to those in gastro-duodenal patients. The aim of this study was to determine H. pylori vacAand cagA genotypes in Thai patients with gastro-duodenal and hepatobiliary diseases.Methodology: H. pyloriDNA was extracted from samples from gastric biopsies of gastro-duodenal patients (n=100) and from bile samples of hepatobiliary patients (n=80). The vacA and cagA genotypes were performed via polymerase chain reaction (PCR) followed by DNA sequencing.Results: The vacA m1 was found in Thai hepatobiliary patients (90%) at a higher rate compared with gastro-duodenal patients (50%).The combined vacA s1a+c/m1 were mostly found in Thai gastro-duodenal and hepatobiliary patients. The cagA gene was detected in 94% of patients with gastro-duodenal diseases compared with 28.8% in those with hepatobiliary diseases (p<0.05). On the other hand, the Western type cagA was more prominent among hepatobiliary patients (100%) than gastro-duodenal patients (57.4%), and this type was grouped into same cluster with Thai gastro-duodenal patients via phylogenetic analysis.Conclusions: Based on vacA and cagA analysis, we conclude that infection with H. pyloriin gastro-duodenal and hepatobiliary patients may be caused by the different H. pyloristrains.engMahidol UniversityH. pylorivacAcagAgastro-duodenal diseaseshepatobiliary diseasesGenetic characterization of Helicobacter pylori vacA and cagA genes in Thai gastro-duodenal and hepatobiliary patientsOriginal ArticleThe Journal of Infection in Developing Countries10.3855/jidc.8126